IBL In vitro plant breeding

Technical information Practicalities

Type of facility: Laboratory - micropropagation by organogenesis and somatic embryogenesis

Keywords: micropropagation, somatic embryogenesis, organogenesis, silviculture, tissue culture, tree breeding

Who would find this useful? Researchers interested in micropropagation of forest tree and application of tissue cultures technology to forest tree improvement.

About the facility: The Laboratory of Tissue Culture is a part of the Department of Silviculture and Genetics  of the Polish Forest Research Institute and works on micropropagation by organogenesis and somatic embryogenesis of broadleaved species (Prunus avium and Betula spp.) We are also ready to work with the other species: Populus tremula x tremuloides, Daphne cneorum, Prunus fruticosa, Picea abies, Larix decidua. Somatic embryogenesis: Picea abies, Larix decidua, Abies alba.

The laboratory works on common projects on molecular genetics and applied tree breeding. The laboratory is currently providing service and research in local projects financed by the Polish State Forest. The projects now are oriented mostly on micropropagation of broadleaved species (Prunus avium and Betula spp.).

Using the in vitro propagated plants - two experimental trials have been established in the last year. The laboratory is also ready to work with other species.

The laboratory has experience in previous projects concerning:

  • organogenesis: Populus tremula x tremuloides, Daphne cneorum, Prunus fruticosa, Picea abies, Larix decidua
  • somatic embryogenesis: Picea abies, Larix decidua, Abies alba.

The IBL Tissue culture laboratory has cooperated with Polish, and European as well as Canadian laboratories. For commercial scale our lab produced selected poplar clones for a Swedish company.


composite image of work at the IBL tissue culture laboratoryImage: IBL. Fig. 1. Mature zygotic embryo of Norway spruce (Picea abies) (explant) Fig. 2 a,b. Initiation of embryogenic callus on mature zygotic embryo of Norway spruce (Picea abies). Fig. 3 a,b. Proembryo of Norway spruce (Picea abies). Fig. 4. Embryogenic callus multiplication: (a) silver fir (Abies alba), (b) Norway spruce (Picea abies) on solid medium, c) Norway spruce (Picea abies) on filter paper placed on solid medium Fig. 5. Cotyledonary somatic embryos of (a) European larch (Larix decidua), and (b) Norway spruce (Picea abies).

What does the TA programme offer?

  • Research stays will usually last from three weeks to 3 months.
  • Training on laboratory equipment and protocols

More information

Location: Sękocin Stary, Poland

Technical information about the facility

Contact details/practical information

Visit the IBL website